PREPARATION OF URINE SAMPLES

Forensic Science Project

Stock Solution

To 1 L of distilled water add:

• 3 g NaCl
• 3 g Ammonium oxalate
• 3 g Potassium phosphate

For students to perform Part 1 to Part 6 of the activity for the first time, the following solution is to be made:

To the 1 L stock solution add:

• 2 drops of 1 M HCl
• 1 aspirin tablet
• 1 g glucose
• 1 g albumin powder
• 5 g urea
• blood cells ( Obtain fresh from butcher/meat department)

Sample Solutions

To a 1 L stock solution for each sample add the following:

Sample #1

• 24 ml 0.1M NH3
• 1 g glucose

Sample #2

• 5 g urea
• 1 g albumin powder

Sample #3

• 1 g glucose
• 1 g albumin powder
• blood cells

Sample #4

• 1 aspirin
• 2 drops of 3M HCL

Note

• Solutions will keep for about a week in the fridge.
• Adjust pH as required.
• Fresh blood cells should be added to appropriate samples each time the sample is used as they tend to lyse in solution.
• Blood obtained from butcher or meat department usually contains few whole blood cells. Centrifuge blood and pour off liquid. Resuspend cells in a small portion of "urine" and add to sample.

Summary Key of Samples

RELEVANT LINKS

Return to Teacher Urine Analysis Page

Return to Forensic Science Introduction Page